Copy Link
Add to Bookmark
Report
dictyNews Volume 04 Number 11
CSM News
Electronic Edition
Volume 4, number 11
March 25, 1995
Please submit abstracts of your papers as soon as they have been
accepted for publication by sending them to CSM-News@worms.cmsbio.nwu.edu.
Back issues of CSM-News, the CSM Reference database and other useful
information is available by anonymous ftp from worms.cmsbio.nwu.edu
[165.124.233.50], via Gopher at the same address, or by World Wide Web
at the URL "http://worms.cmsbio.nwu.edu/dicty.html"
===========
Abstracts
===========
Cloning and Expression in Escherichia coli of a cDNA Encoding a
Developmentally Regulated Ca2+-binding Protein from Dictyostelium
discoideum
Barrie Coukell, John Moniakis and Avie Grindberg
Department of Biology, York University, 4700 Keele St., North York,
Ontario, CANADA, M3J 1P3
FEBS Lett., in press
Abstract:
We have cloned a full-length cDNA from Dictyostelium discoideum
which encodes a new Ca2+-binding protein. The deduced protein (termed
CBP1) is composed of 156 amino acids and contains four consensus
metal-ligating loop sequences found in helix-loop-helix motifs of many
Ca2+-binding proteins. When expressed in bacteria as a GST fusion
protein, CBP1 binds Ca2+ in a 45Ca2+ overlay assay. CBP1 exhibits
little amino acid sequence homology with Dictyostelium calmodulin or
calfumirin-1 (CAF-1) except in the putative Ca2+-binding regions.
Moreover, unlike calmodulin and CAF-1 expression, CBP1 mRNA is
expressed preferentially during the multicellular stages of
development.
-----------------------------------------------------------------------
[End CSM-News, volume 4, number 11]
